Vignettes weaving together the stories of six individuals in the old West at the end of the Civil War. Following the tales of a sharp-shooting songster, a wannabe bank robber, two weary traveling performers, a lone gold prospector, a woman traveling the West to an uncertain future, and a motley crew of strangers undertaking a carriage ride.
When asked about the Ghost Riders song he sings, Gene Autry tells this legend: Gene is about to resign as an investigator for the county attorney and go into the cattle business with his pal Chuckawalla Jones but decides instead to help Anne Lawson clear her father, rancher Ralph Lawson, of a false murder charge. He looks for the three witnesses who can testify that Lawson shot only in self defense in killing a gambler, but the witnesses are terrorized by another gambler, town boss Rock McCleary, who shoots witness Pop Roberts Morgan. Fatally wounded, Pop gives Gene the information needed to clear Lawson, then dies crying the "Ghost Riders" are coming for him. Gene then heads for a showdown with McCleary.
James Kincaid controls the local water supply and plans to do away with the other ranchers. Government agent Sandy Saunders arrives undercover to investigate Kincaid's land swindle scheme, and win the heart of one of his victims, Fay Denton.
Two peanut vendors at a rodeo show get in trouble with their boss and hide out on a railroad train heading west. They get jobs as cowboys on a dude ranch, despite the fact that neither of them knows anything about cowboys, horses, or anything else.
Roy returns home to fine a range feud between the cattlemen and the sheepmen. When his friend is killed he finds the rifle had a defective pin. He learns the rifle belongs to a ranch hand named Barker and that a third party has caused the feud. When he captures outlaws trying to blow up a dam, he claims Barker was the killer. But Barker has switched rifles and the outlaws now accuse Roy and Roy finds himself in trouble.
On vacation at his ranch, western actor Roy quickly finds himself involved with a horse rustling operation and a boy ward of one of the rustlers, leading to the kidnapping of Roy's trick horse Trigger by the gang with a demand for ransom.
A cowboy helps a pretty young woman and her father in their fight against land-grabbers who are trying to swindle them out of their cattle ranch.
It's cattlemen versus sheepmen and Trigger Gargan appears to be the leader of the gang causing the trouble. But unknown to Ranger Tex Lawrence, the respected town citizen Barrow is the boss and is tipping off the gang as to the Ranger's activities.
To bring water to their valley, ranchers have raised money to build a dam. When that money is stolen, Allison suggests the ranchers sell their stock to a friend of his thereby getting the money needed to complete the dam. Roy has a clue that Allison was involved in the robbery and is out to get control of the valley. So Roy and the boys try to delay the sale of the stock while they look for proof against Allison.
Gene Autry and his sidekick Frog look into a phony oil scam being perpetrated on a mission orphanage.
Dorothy, and her big city lawyer boyfriend, return to the Lazy 'B' ranch to read her late father's will. For Dorothy to inherit everything, she must stay on the ranch for 5 years. If she does not, everything goes to Buck, who is the manager. She does not like Buck, so she makes a deal with the wrong people for cattle and then the outlaws go to the ranch to get the $10,000 from her. But Buck is on the job.
A singing cowboy roams the Wild West with his sidekick, dancing horse and fancy wardrobe.
A singing cowboy and his sidekick encounter misunderstandings and rodeo havoc as they try and save a man and daughter from con men.
A cattle buyer, a federal agent and a newswoman snip a railroad plot.
Fur theives are looting the traps on the ranch where Roy is foreman and they have murdered one of Roy's friends.
A wrongfully-imprisoned man becomes determined to find who was responsible for the death of a local sheriff.
Bad guys plot to trick a newly arrived Eastern girl out of a ranch which belongs to her infant ward. Roy, of course, saves the ranch for the girl. Songs include "I'm Headin's for the Home Corral," "He's a No Good Son of a Gun," "Sandman Lullaby," "Song of the San Joaquin," and "I'm a Cowboy Rockefeller."
Rancher Autry takes a job singing on the radio to aid farmers and ranchers whose lands were destroyed by raging floods. Blaming crooked politicians, he goes to Washington and tries to put through a food control bill and finds he has a lot to learn. In this classic release, Gene introduces his immortal theme song, "Back in the Saddle Again," which has gone on to become a piece of American History.
Caxton has broken out of prison and Eddie has been sent to bring him in. Caxton is known by the polka dot band on his hat and Eddie has Soapy wear one like it. This gets Soapy arrested as soon as he rides into town but it leads Eddie to Varney and he realizes Varney will lead him to Caxton.
Scanlon is pulling off a land swindle by selling lots in a ghost town claiming the power company is bringing in a line. As a bonus he throws in shares in a worthless gold mine. Gene is on to Scanlon and tries to get him to buy back the deeds by salting the mine with gold. But when a new vein is really discovered Gene has to stop the sales but is trapped in the mine by Scanlon's men.